The NRL will cut 25 percent of staff in a business restructure designed to save $50 million as they count the cost of COVID-19.
Chief executive Andrew Abdo announced the cuts in a teleconference on Monday with staff to meet with their direct managers this week to learn of their future.
Adbo said the organisation would lose "some very good people during this process".
"Talented people with a genuine love and passion for our game who have all contributed in some way," he said. "This is painful but necessary process to ensure we survive.
"I have genuine empathy for all employees affected and we will listen to their feedback."
The restructure comes after the executive team was whittled from 11 to eight, with employees to be consulted over the latest changes "over the coming weeks".
Chief operating officer Nick Weeks and chief of corporate affairs Liz Deegan were made redundant at the start of September, while former CEO Todd Greenberg and chief financial officer Tony Crawford exited earlier this year.
The simplified system will see staff split into seven areas down from 10: competitions, partnerships, fan experience, finance, technology and operations, risk, integrity and performance, brand, media and communications and participation and community.
Graham Annesley will remain head of competitions, with Jaymes Boland-Rudder taking over as head of partnerships, including government.
Alex Alderson (fan experience), Richard Gardham (finance), Eleni North (risk, integrity and performance) and Daniel Meers (communications) have been announced as the other new division managers.
"Our business, like so many others, has been hit by a hurricane called COVID which caused substantial damage. Our strategy moving forward is to stabilise, renovate and grow,'' Abdo said. "In order to stabilise, we must secure revenues and reduce our expenses to ensure a strong foundation.
"Then we will renovate by considering ways to make our products more entertaining and dynamic for our fans.
"Finally, we will develop bold plans for growth, looking at new products, new markets and how we can grow the game internationally."
The job cuts are expected to be finalised by Friday.