The Matildas will be aiming aim to show that Saturday's 3-1 win against the same opponents was not a one-off and, in turn, give Gustavsson the first back-to-back victory of his tenure.
Both Debinha and Marta were restricted to 20 minutes in the first of two friendlies as coach Pia Sundhage juggled a squad going through a period of change along with the more immediate concern of jet lag.
But in their short time on the field, the pair's influence was plain to see and Gustavsson expects them to start from the get-go.
"I think because of the travel and jet lag they have probably eased her [Marta] and Debinha into training to protect them a little bit," Gustavsson said.
"I know they [Brazil] are in a phase where they are experimenting and rebuilding with young players.
"I would be surprised if Marta and Debinha didn't both start in this game.
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"Honestly, I hope they do because in these games we want to play the best teams to make sure we get exposed to quality players."
The presence of Debinha and Marta, a five-time FIFA world player of the year winner, will undoubtedly require greater defensive resolve from the Australians.
Gustavsson was concerned at some of the areas in which his team turned the ball over on Saturday which allowed Brazil to break quickly and ask questions of Australia's back four.
Sundhage's side were restricted to just four shots on target, but most of their opportunities came as a consequence of the Matildas surrendering possession cheaply.
"Brazil is going to target our centre-backs and try to get in behind," he said.
"We need to have good positioning when we get dispossessed but also our initial reaction [must be better] so that we can stop that transition before it even happens.
"Then, if it happens, how can we deal with those balls in behind?
"They had some really good pace and delivery to get in behind and we saw that when they got one-on-one with Lydia and we obviously want to avoid that as much as possible."