Burrow was a game-time decision after dealing with the ailing calf throughout the week. He did not practice Thursday and was a limited participant in practices Friday and Saturday.
Burrow warmed up and threw passes to receivers before going back to the locker room to get ready for Monday night's game.
Cincinnati has dropped its first two games for the second straight season and is looking to avoid its first three-game losing streak since 2020, Burrow's rookie season.
Earlier in the week, Burrow said the early season defeats played a role in the urgency he felt to play against the Rams in a rematch of Super Bowl LVI, which Los Angeles won.
"It's in my head, for sure," Burrow said Thursday. "I'm thinking about it."
For the Rams, breakout rookie receiver Puka Nacua, who had been questionable with an oblique injury, was also active for the game.