Kucherov got hurt chasing a puck during the third period Oct. 16 at Washington. The high-scoring forward appeared to be injured in the groin-abdominal area.
"He's ready to go," Lightning coach Jon Cooper said after Thursday's morning skate.
Tampa Bay captain Steven Stamkos did not participate in the morning skate due to an undisclosed issue but played against the Flames.
Kucherov sat out the abbreviated 2020-21 regular season following hip surgery, but he returned for the playoffs and had a league-leading 32 points (eight goals, 24 assists) in 23 games as the Lightning won their second consecutive Stanley Cup.
"We're super excited about it," Lightning defenseman Victor Hedman said. "He just brings a different element to our game and is going to make us a lot better."
Tampa Bay (22-8-5) entered Thursday with an NHL-best 49 points.