England have called up Henry Arundell and centre Henry Slade for the Six Nations clash with Italy on Sunday at Twickenham after both have struggled with injuries.
Both players were included in England coach Steve Borthwick's 36-player squad announced on Monday.
Winger Cadan Murley was also left out, having not made the cut for the match against Scotland.
The Borthwick era got off to a inauspicious start as a tenacious Scotland took a 29-23 victory at Twickenham in a rip-roaring Six Nations opener.
Courtney Lawes will again be in the camp but unlikely to feature against Italy as he continues rehabilitation work from a calf injury.