- Sam Kerr (32')
- Charlotte Grant (67')
Goals from Sam Kerr and Charlotte Grant earned Australia a 2-0 win over England at a rainy Brentford Community Stadium on Tuesday to end a 30-match unbeaten run for Sarina Wiegman's team.
With 100 days to go before co-hosting the Women's World Cup with New Zealand, the Matildas returned to winning ways after their friendly defeat by Scotland on Friday thwarted a seventh straight win.
England's unbeaten run of 26 wins and four draws had included winning the European Championship last summer.
"I think most of all they [Australia] were very efficient. We gave them chances," Wiegman told ITV. "In possession we were too slow. You need dynamic movement to stretch them, we didn't do that well enough."
Kerr opened the scoring in the 32nd minute capitalising on a poor header back to keeper Mary Earps by defender Leah Williamson to coolly slot home, despite appearing to be offside as there was no VAR in place.
"England's such a big footballing nation, hopefully there are some happy Aussies, but we're not getting ahead of ourselves," Kerr said.
Australia's counter-attacking play paid off and Grant doubled the lead in the 67th minute with a header that bounced off Williamson, who had a night to forget, and into the net.
"We looked like we lacked ideas on the ball, and we got punished. It was my mistake for first goal, and they were ruthless on counter," Williamson said.
"I'm not one for dwelling on personal things, that first goal has made it worse for me but the whole teams feels really rubbish about losing. It hurts.
"Absolutely not a setback [for the World Cup]. For us to lose tonight gives us a bit of fire."
The Lionesses pressed but could not find a way past a solid Australian defence, as they lost for the first time under Wiegman's tenure.
"This is the first time we lose, but we always learn," Wiegman added. "We have to improve to be at our best at the World Cup. Every game is for learning, and this is a big one."
England beat Brazil in a 4-2 penalty shootout victory last Thursday in front of 83,132 fans at Wembley Stadium.
Australia play France in a friendly on July 14, before their World Cup opener against Ireland six days later. England kick off their World Cup campaign against Group D opponents Haiti on July 22.
- Natalie Simon